3AJD
Crystal structure of ATRM4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.128, 40.108, 90.559 |
| Unit cell angles | 90.00, 102.75, 90.00 |
Refinement procedure
| Resolution | 33.180 - 1.270 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ixk |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.041 | 0.335 |
| Number of reflections | 72008 | |
| <I/σ(I)> | 30 | 0.313 |
| Completeness [%] | 98.0 | 90.8 |
| Redundancy | 3.3 | 3.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 100MM TRIS-HCL (PH 7.4), 6 % V/V 2-PROPANOL, 200MM MGCL2, 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
