3AHQ
hyperactive human Ero1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-04 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.90 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 54.962, 140.730, 144.855 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.270 - 2.350 |
| R-factor | 0.23938 |
| Rwork | 0.237 |
| R-free | 0.28231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDN ENTRY 1RP4 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.652 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.500 | 2.470 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.054 | 0.439 |
| Number of reflections | 23814 | |
| <I/σ(I)> | 21 | 4.4 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 8% PEG4000, 50mM imidazole pH8.0, VAPOR DIFFUSION, temperature 293K |
