3AEE

Crystal structure of porcine heart mitochondrial complex II bound with Atpenin A5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-24
Detector	ADSC QUANTUM 210
Wavelength(s)	0.80000
Spacegroup name	P 21 21 21
Unit cell lengths	70.751, 83.841, 293.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.960 - 3.220
R-factor	0.235
Rwork	0.232
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zoy
RMSD bond length	0.007
RMSD bond angle	1.110
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.079	0.488
Number of reflections	27959
<I/σ(I)>	15.657	2.4
Completeness [%]	95.7	97.4
Redundancy	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	25mM HEPES-NAOH, 5% PEG 4000, 200mM Sucrose, 100mM NaCl, 10mM CaCl2, 0.5mM EDTA, 3% 1,6-haxanediol, 0.5% n-decyl-beta-D-maltoside, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K