3ABD
Structure of human REV7 in complex with a human REV3 fragment in a monoclinic crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.870, 50.009, 107.275 |
| Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.900 |
| R-factor | 0.18939 |
| Rwork | 0.187 |
| R-free | 0.24074 |
| Structure solution method | MIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.689 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | 0.268 |
| Number of reflections | 35667 | |
| <I/σ(I)> | 14.1 | 3.7 |
| Completeness [%] | 97.3 | 83.1 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25% PEG 2000 MME, 0.8M sodium formate, 0.1M tris-HCl pH 7.5,, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
