3A4T
Crystal structure of aTrm4 from M.jannaschii with sinefungin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2008-04-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 170.259, 40.097, 85.951 |
Unit cell angles | 90.00, 110.84, 90.00 |
Refinement procedure
Resolution | 42.450 - 2.300 |
R-factor | 0.18551 |
Rwork | 0.181 |
R-free | 0.26756 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3A1X |
RMSD bond length | 0.027 |
RMSD bond angle | 2.284 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.450 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.022 | 0.564 |
Number of reflections | 24668 | |
<I/σ(I)> | 19.5 | 0.488 |
Completeness [%] | 1.0 | 100 |
Redundancy | 3.6 | 2.04 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 100mM Tris-HCl buffer (pH 7.4) , 200mM MgCl2 ,25% PEG 3350, 10mM cytidine, 3mM sinefungin, VAPOR DIFFUSION, HANGING DROP, temperature 293K |