36WL

Mevalonate kinase from Saccharomyces cerevisiae with geranyl pyrophosphate (GPP) bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	83.450, 83.450, 262.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.540 - 2.050
R-factor	0.1789
Rwork	0.178
R-free	0.19780
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	0.861
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.590	2.120
High resolution limit [Å]	2.050	2.050
Number of reflections	58995	4515
<I/σ(I)>	12.4
Completeness [%]	100.0	100
Redundancy	6.6	6.9
CC(1/2)	0.997	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM MES pH 6.0, 900-1100 mM sodium potassium tartrate