35UH
Crystal structure of TFPI K2 domain in complex with TFPI-23 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.669, 101.372, 119.275 |
| Unit cell angles | 101.83, 92.27, 96.78 |
Refinement procedure
| Resolution | 44.200 - 3.110 |
| R-factor | 0.2282 |
| Rwork | 0.226 |
| R-free | 0.27330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.804 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.530 | 3.190 |
| High resolution limit [Å] | 3.110 | 3.110 |
| Rmerge | 0.149 | 1.493 |
| Rmeas | 0.211 | 2.109 |
| Number of reflections | 559983 | 4387 |
| <I/σ(I)> | 5 | |
| Completeness [%] | 98.5 | |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM ammonium acetate, 17 % PEG 1000, 100 mM bis-tris pH 5.5 |
