35VB
Crystal structure of KRAS G13C (GMPPNP-bound) in complex with RAS-binding domain (RBD) and cysteine-rich domain (CRD) of BRAF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 166.788, 166.788, 166.788 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.740 - 2.850 |
| R-factor | 0.2115 |
| Rwork | 0.210 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.642 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.740 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.110 | 2.017 |
| Rpim | 0.044 | 0.816 |
| Number of reflections | 17527 | 2504 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7 |
| CC(1/2) | 0.999 | 0.316 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.8 M sodium acetate and 0.1 M Bis-Tris propane, pH 7.0 |
