32QP
Human TRIM21 PRYSPRY domain in complex with PRLX-93936
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-31 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.613, 59.613, 169.577 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.810 - 2.100 |
| R-factor | 0.20734 |
| Rwork | 0.206 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.466 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.240 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 18781 | 1508 |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 25.9 | |
| CC(1/2) | 1.000 | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 4.3 M Sodium Chloride 0.1 M Hepes pH8 |
