31RF
POLQ-Taq chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2021-12-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.415, 80.315, 95.871 |
| Unit cell angles | 90.00, 93.96, 90.00 |
Refinement procedure
| Resolution | 61.510 - 1.980 |
| R-factor | 0.223 |
| Rwork | 0.222 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.510 | 5.370 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 91238 | 4521 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.984 | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 6% PEG3350, 0.1 M PCPT pH 6.5, 0.2M NaCl |
