316D
Selectivity of F8-actinomycin D for RNA:DNA hybrids and its anti-leukemia activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 299 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-01 |
| Detector | MACSCIENCE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 62.770, 62.770, 43.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 3.000 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DDH051 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 24.000 * |
| Data reduction software | ELMS |
| Data scaling software | KUAVST |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 7.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.075 | 0.002 |
| Number of reflections | 1839 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 95.0 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 26 * | PH 7.00, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 299.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | CACODYLATE | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 2.0 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | cacodylate/HCl | 20 (mM) | |
| 4 | 1 | drop | F8AMD | 1.5 (mM) | |
| 5 | 1 | reservoir | MPD | 15 (%) |
