30BG
Structure of the Hepatitis C Virus E2 Core from Genotype 6a in Complex with 1-69 Germline-Reverted Variants of the Broadly Neutralizing Antibody U1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-21 |
| Detector | DECTRIS PILATUS4 X 4M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.849, 79.072, 133.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.610 - 3.310 |
| R-factor | 0.2704 |
| Rwork | 0.268 |
| R-free | 0.32380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.551 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASES (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.610 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.683 | |
| Number of reflections | 9197 | 480 |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 92.2 | |
| Redundancy | 1.7 | |
| CC(1/2) | 0.970 | 0.330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Potassium chloride, and 20% (w/v) PEG 3350 |
