30LM

Mouse cGAS in complex with DNA and ATP, crystallized in presence of Ap4A.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	90
Detector technology	PIXEL
Collection date	2025-09-30
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.873128
Spacegroup name	P 21 21 21
Unit cell lengths	78.030, 98.940, 143.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.680 - 2.700
R-factor	0.2306
Rwork	0.229
R-free	0.26330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.753
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (v1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.750	2.860
High resolution limit [Å]	2.700	2.700
Number of reflections	31264	4968
<I/σ(I)>	11.37	1.44
Completeness [%]	99.9
Redundancy	13.43
CC(1/2)	0.998	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Solution of 50 mM Calcium acetate, 100 mM Imidazole pH 8.0, 30-35% (v/v) 2-Ethoxyethanol ; added to 250 uM cGAS protein, 300 uM DNA, 2 mM Ap4A, in 20 mM Tris-HCL pH 7.5, 0.5 mM TCEP, 150 mM NaCl, 5 mM MgCl2 in a 1 to 1 or 1 to 2 ratio.