2ZZS
Crystal structure of cytochrome c554 from Vibrio parahaemolyticus strain RIMD2210633
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 84.949, 87.612, 103.847 |
Unit cell angles | 71.50, 72.98, 83.68 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.19519 |
Rwork | 0.193 |
R-free | 0.24213 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gdv |
RMSD bond length | 0.018 |
RMSD bond angle | 1.777 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.063 | 0.205 |
Number of reflections | 223962 | |
<I/σ(I)> | 14.1 | |
Completeness [%] | 94.5 | 77.9 |
Redundancy | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 10mM sodium phosphate buffer (pH 7.0), 0.1M phosphate-citrate (pH 4.2), 0.2M NaCl, 20%(w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |