2ZZP

The crystal structure of human Atg4B(C74S)- LC3(1-124) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	90
Detector technology	CCD
Collection date	2007-11-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	49.250, 93.146, 101.999
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.440 - 2.050
R-factor	0.208
Rwork	0.208
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z0e
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.079	0.268
Number of reflections	27207
<I/σ(I)>	11
Completeness [%]	90.8	73.8
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	20% PEG 3350, 0.1M sodium citrate, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K