2ZZA
Moritella profunda Dihydrofolate reductase complex with NADP+ and Folate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-13 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.598, 77.458, 159.819 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.330 - 2.000 |
| Rwork | 0.201 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dds |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.319 |
| Number of reflections | 21062 | |
| <I/σ(I)> | 2.82 | 2.82 |
| Completeness [%] | 85.5 | 87.2 |
| Redundancy | 5.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 0.2M Ammonium acetate, 0.1M sodium citrate, 15% PEG4000, 0.1 Hexammine Cobalt (III) chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
