2ZZ7
Orotidine Monophosphate Decarboxylase K72A mutant complexed with BMP (produced from 6-Iodo-UMP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Detector technology | CCD |
| Collection date | 2004-03-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.933, 103.290, 73.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.640 - 1.580 |
| R-factor | 0.15251 |
| Rwork | 0.152 |
| R-free | 0.17080 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | ODCase from M. thermoautotrophicum |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.609 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.057 | 0.301 |
| Number of reflections | 30641 | |
| <I/σ(I)> | 33.2 | 7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 1.2-1.36M Sodium Citrate, 5% dioxiane, pH 6.0-8.5, VAPOR DIFFUSION, temperature 298K |
