2ZZ6
Covalent complex of orotidine monophosphate decarboxylase from M. thermoautotrophicum with 6-azido-UMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Detector technology | CCD |
| Collection date | 2004-03-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.931, 73.692, 59.147 |
| Unit cell angles | 90.00, 119.31, 90.00 |
Refinement procedure
| Resolution | 27.440 - 1.660 |
| Rwork | 0.162 |
| R-free | 0.18700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1x1z |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.060 | |
| Number of reflections | 50234 | |
| <I/σ(I)> | 22.6 | 4.5 |
| Completeness [%] | 98.3 | 97 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 1.2-1.36M Sodium Citrate, 5% dioxiane, pH 6.0-8.5, VAPOR DIFFUSION, temperature 298K |
