2ZYF
Crystal structure of homocitrate synthase from Thermus thermophilus complexed with magnesuim ion and alpha-ketoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 135.465, 135.465, 126.612 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.510 - 2.150 |
| R-factor | 0.20469 |
| Rwork | 0.203 |
| R-free | 0.23152 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ztj |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.112 | 0.546 |
| Number of reflections | 37714 | |
| <I/σ(I)> | 40.8 | 4.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.8 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES-NaOH (pH6.0), 1.6M Ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
