2ZY9
Improved crystal structure of magnesium transporter MgtE
Experimental procedure
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.364, 76.527, 160.037 |
Unit cell angles | 90.00, 102.17, 90.00 |
Refinement procedure
Resolution | 26.713 - 2.940 |
R-factor | 0.257 |
Rwork | 0.255 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ABC |
RMSD bond length | 0.004 |
RMSD bond angle | 0.807 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.990 |
High resolution limit [Å] | 2.940 | 2.940 |
Number of reflections | 33428 | |
<I/σ(I)> | 12.5 | 2.4 |
Completeness [%] | 95.1 | 84.4 |
Redundancy | 3.8 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 8% PEG 4000, 0.2M MgCl2, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |