2ZWG

Crystal structure of the copper-bound tyrosinase in complex with a caddie protein from streptomyces castaneoglobisporus obtained by soaking the deoxy-form crystal in dioxygen-saturated solution for 12 hours

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-07
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.8000
Spacegroup name	P 21 21 2
Unit cell lengths	65.240, 97.760, 55.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.320
R-factor	0.1794
Rwork	0.179
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1WX3
RMSD bond length	0.011
RMSD bond angle	0.028
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.370
High resolution limit [Å]	1.320	1.320
Rmerge	0.047	0.423
Number of reflections	78096
<I/σ(I)>	23.8	2.2
Completeness [%]	93.7	97.7
Redundancy	4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	297	PEG 3350, SODIUM NITRATE, HEPES, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 297K