2ZW3
Structure of the connexin-26 gap junction channel at 3.5 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-17 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.617, 111.245, 155.387 |
| Unit cell angles | 90.00, 114.04, 90.00 |
Refinement procedure
| Resolution | 22.000 - 3.500 |
| R-factor | 0.33755 |
| Rwork | 0.337 |
| R-free | 0.35123 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.352 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 141.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.085 | 0.378 |
| Number of reflections | 31138 | |
| <I/σ(I)> | 26.1 | 3.1 |
| Completeness [%] | 94.1 | 94.9 |
| Redundancy | 11.2 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 16-18% PEG 200, 0.1M Sodium phosphate, 0.1M Sodium chloride, 0.01M DTT , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
