2ZVX
Structure of a BPTI-[5,55] variant containing Gly/Val at the 14/38th positions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-10-04 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.962, 40.844, 54.829 |
| Unit cell angles | 90.00, 100.55, 90.00 |
Refinement procedure
| Resolution | 53.910 - 1.090 |
| R-factor | 0.1787 |
| R-free | 0.21230 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | 2zjx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.910 | |
| High resolution limit [Å] | 1.090 | 1.090 |
| Rmerge | 0.042 | 0.042 |
| Number of reflections | 35190 | |
| Completeness [%] | 96.0 | |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl, pH8.5, 0.2M Lithium chloride, 30% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
