2ZV8
Lyn Tyrosine Kinase Domain-AMP-PNP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Wavelength(s) | 1.54180 |
| Spacegroup name | H 3 |
| Unit cell lengths | 127.239, 127.239, 54.875 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.740 - 2.700 |
| R-factor | 0.20134 |
| Rwork | 0.199 |
| R-free | 0.24493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qpd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.153 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.900 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 9082 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 23% polyethylene glycol 3350, 0.1M NaCl, 0.1M Na-Hepes pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
