2ZUQ
Crystal structure of DsbB-Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-19 |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.90 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 269.307, 51.480, 125.845 |
| Unit cell angles | 90.00, 106.89, 90.00 |
Refinement procedure
| Resolution | 45.640 - 3.300 |
| R-factor | 0.27949 |
| Rwork | 0.275 |
| R-free | 0.35119 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1igt |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.492 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.510 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.086 | 0.865 |
| Number of reflections | 25296 | |
| <I/σ(I)> | 11.7 | 1.7 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 15% PEG3350, pH 7, VAPOR DIFFUSION, temperature 293K |
