2ZT8
Crystal structure of human Glycyl-tRNA synthetase (GlyRS) in complex with Gly-AMP analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Collection date | 2008-05-20 |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 119.600, 138.258, 132.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.350 |
| Rwork | 0.221 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pme |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.060 | 0.164 |
| Number of reflections | 16000 | |
| <I/σ(I)> | 32.6 | 7.9 |
| Completeness [%] | 95.6 | 71.4 |
| Redundancy | 6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 25mM Tris-HCl, 150mM NaCl, 10mM MgCl2, 3.8-4.0M Sodium Formate, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
