2ZSW
Crystal structure of H-2Kb in complex with the Q600Y variant of JHMV epitope S598
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-10-29 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 |
Unit cell lengths | 66.711, 90.147, 91.969 |
Unit cell angles | 81.11, 70.58, 68.25 |
Refinement procedure
Resolution | 27.000 - 2.800 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.25284 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g7q |
RMSD bond length | 0.013 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.000 | 2.910 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.101 | 0.303 |
Number of reflections | 44395 | |
<I/σ(I)> | 10 | 2.1 |
Completeness [%] | 95.8 | 85.6 |
Redundancy | 2.3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1M cacodylate, 13% PEG 8K, 0.2M Ca(OAc)2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |