1G7Q

CRYSTAL STRUCTURE OF MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND MUC1 VNTR PEPTIDE SAPDTRPA

Summary for 1G7Q

• Entry DOI: 10.2210/pdb1g7q/pdb
• Related: 1VAC 2VAA
• Descriptor: H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, K-B ALPHA CHAIN, BETA-2 MICROGLOBULIN, MUCIN 1, TRANSMEMBRANE, ... (6 entities in total)
• Functional Keywords: mhc class i, h-2kb, muc1, vntr, mucin, immune system
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 3
• Total formula weight: 44959.29

Authors

Apostolopoulos, V.,Yu, M.,Corper, A.L.,Teyton, L.,Pietersz, G.A.,McKenzie, I.F.,Wilson, I.A. (deposition date: 2000-11-13, release date: 2002-07-17, Last modification date: 2020-07-29)

Primary citation

Apostolopoulos, V.,Yu, M.,Corper, A.L.,Teyton, L.,Pietersz, G.A.,McKenzie, I.F.,Wilson, I.A.
Crystal structure of a non-canonical low-affinity peptide complexed with MHC class I: a new approach for vaccine design.
J.Mol.Biol., 318:1293-1305, 2002

PubMed Abstract: Peptides bind with high affinity to MHC class I molecules by anchoring certain side-chains (anchors) into specificity pockets in the MHC peptide-binding groove. Peptides that do not contain these canonical anchor residues normally have low affinity, resulting in impaired pMHC stability and loss of immunogenicity. Here, we report the crystal structure at 1.6 A resolution of an immunogenic, low-affinity peptide from the tumor-associated antigen MUC1, bound to H-2Kb. Stable binding is still achieved despite small, non-canonical residues in the C and F anchor pockets. This structure reveals how low-affinity peptides can be utilized in the design of novel peptide-based tumor vaccines. The molecular interactions elucidated in this non-canonical low-affinity peptide MHC complex should help uncover additional immunogenic peptides from primary protein sequences and aid in the design of alternative approaches for T-cell vaccines.

PubMed: 12083518
DOI: 10.1016/S0022-2836(02)00196-1

Experimental method

X-RAY DIFFRACTION (1.6 Å)