2ZSV
Crystal structure of H-2Kb in complex with JHMV epitope S598
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.487, 89.539, 89.932 |
| Unit cell angles | 90.00, 111.68, 90.00 |
Refinement procedure
| Resolution | 24.400 - 1.800 |
| R-factor | 0.21222 |
| Rwork | 0.210 |
| R-free | 0.24975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g7q |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.618 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.460 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.046 | 0.385 |
| Number of reflections | 90277 | |
| <I/σ(I)> | 18 | 2 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 294 | 0.1M cacodylate, 16% PEG 8K, 0.2M Ca(OAc)2, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
