2ZSU
Crystal structure of spermidine synthase from Pyrococcus horikoshii OT3, P1 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-17 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.617, 81.842, 96.653 |
| Unit cell angles | 99.81, 103.13, 107.85 |
Refinement procedure
| Resolution | 27.920 - 2.200 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e5w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.094 | 0.398 |
| Number of reflections | 90319 | |
| <I/σ(I)> | 7.5 | 2.77 |
| Completeness [%] | 95.9 | 92.9 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.3 | 295 | 0.2M NH4Cl, 20% w/v PEG 3350, pH 6.3, microbatch, temperature 295K |
