2ZR2
Crystal structure of seryl-tRNA synthetase from Pyrococcus horikoshii complexed with ATP
Replaces: 2DQ1Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2005-06-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.656, 120.499, 126.777 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.520 - 2.800 |
Rwork | 0.192 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dq0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.580 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.062 | 0.612 |
Number of reflections | 37201 | |
<I/σ(I)> | 32.3 | 3.2 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.6M ammonium sulfate, 0.1M magnesium acetate, 0.1M Hepes-NaOH pH 7.5, 2mM ATP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |