2ZPP
Neutron crystal structure of cubic insulin at pD9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | NUCLEAR REACTOR |
| Source details | OTHER |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-22 |
| Detector | BIX-3 |
| Wavelength(s) | 2.9 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 79.040, 79.040, 79.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.222 |
| Rwork | 0.221 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b2g |
| RMSD bond length | 0.008 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.145 | 0.290 |
| Number of reflections | 3747 | |
| <I/σ(I)> | 4.72 | |
| Completeness [%] | 98.9 | 98.1 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 9 | 298 | 0.2M sodium phosphate, 10mM 3NaEDTA, pH 9.0, MICRODIALYSIS, temperature 298K |
