2ZPA

Crystal Structure of tRNA(Met) Cytidine Acetyltransferase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-01-30
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9791,0.9797,0.9645
Spacegroup name	P 21 21 21
Unit cell lengths	61.298, 100.990, 263.116
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.350
Rwork	0.235
R-free	0.27350
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.918
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.056	0.373
Number of reflections	66310
<I/σ(I)>	31.5	3.9
Completeness [%]	96.6	92.1
Redundancy	6.4	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	15% (w/v) PEG 5000 monomethyl ether, 50mM lithium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	15% (w/v) PEG 5000 monomethyl ether, 50mM lithium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K