2ZP1
Structual basis of iodo-tyrosine recognition by engineered archeal tyrosyl-tRNA synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-03 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.201, 102.201, 71.904 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.650 - 1.700 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zh0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.064 | 0.734 |
Number of reflections | 42504 | |
<I/σ(I)> | 43.41 | 2.75 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 13.7 | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 24-28% PEG300, 5-8% PEG8000, 10% Glycerol, 0.1M TRIS, PH8.5, VAPOR DIFFUSION, TEMPERATURE 293K, pH 8.50 |