2ZOC
Crystal structure of recombinant human annexin IV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.223, 55.132, 127.147 |
| Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.22 |
| Rwork | 0.216 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ann |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.130 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.520 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.119 |
| Number of reflections | 38282 | |
| <I/σ(I)> | 13.2 | 6 |
| Completeness [%] | 92.1 | 89.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 6000, calucium chloride, dioxisane, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
