2ZM3
Complex Structure of Insulin-like Growth Factor Receptor and Isoquinolinedione Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 143 |
Detector technology | CCD |
Collection date | 2004-01-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.725, 135.962, 86.380 |
Unit cell angles | 90.00, 114.27, 90.00 |
Refinement procedure
Resolution | 46.370 - 2.500 |
R-factor | 0.2037 |
Rwork | 0.201 |
R-free | 0.25720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqh |
RMSD bond length | 0.003 |
RMSD bond angle | 0.829 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.560 |
High resolution limit [Å] | 2.270 | 2.450 |
Rmerge | 0.096 | 0.462 |
Number of reflections | 60396 | |
<I/σ(I)> | 1.8 | |
Completeness [%] | 90.8 | 75.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris-HCl, pH8, 2M NaCl, 13-16% PEG6K, VAPOR DIFFUSION, HANGING DROP, temperature 277K |