2ZM3
Complex Structure of Insulin-like Growth Factor Receptor and Isoquinolinedione Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 143 |
| Detector technology | CCD |
| Collection date | 2004-01-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.725, 135.962, 86.380 |
| Unit cell angles | 90.00, 114.27, 90.00 |
Refinement procedure
| Resolution | 46.370 - 2.500 |
| R-factor | 0.2037 |
| Rwork | 0.201 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqh |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.829 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.270 | 2.450 |
| Rmerge | 0.096 | 0.462 |
| Number of reflections | 60396 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 90.8 | 75.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris-HCl, pH8, 2M NaCl, 13-16% PEG6K, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
