2ZKS
Structural insights into the proteolytic machinery of apoptosis-inducing Granzyme M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 1W2B |
Synchrotron site | BSRF |
Beamline | 1W2B |
Temperature [K] | 93 |
Detector technology | FLAT PANEL |
Collection date | 2008-03-05 |
Detector | MARRESEARCH Flat Panel |
Wavelength(s) | 0.9789 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 74.671, 74.671, 113.557 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.190 - 2.700 |
R-factor | 0.22609 |
Rwork | 0.223 |
R-free | 0.29308 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2GZC |
RMSD bond length | 0.016 |
RMSD bond angle | 1.880 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 64.680 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.094 |
Number of reflections | 9978 |
<I/σ(I)> | 7.3 |
Completeness [%] | 95.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 20% PEG3350, 0.2M Li2SO4, 0.1M Bicine, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |