2ZKN
X-ray structure of mutant galectin-1/lactose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.925, 87.431, 95.698 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.020 - 1.860 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w6o |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.020 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.051 | 0.184 |
| Number of reflections | 26434 | |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 98.6 | 96.3 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.0M ammonium sulfate, 20mM phosphate buffer, 5mM lactose, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
