2ZKH
Human thrombopoietin neutralizing antibody TN1 FAB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.667, 71.416, 104.433 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.140 - 2.040 |
R-factor | 0.1951 |
Rwork | 0.193 |
R-free | 0.23167 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hrp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.328 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.140 | 2.120 |
High resolution limit [Å] | 2.040 | 2.050 |
Rmerge | 0.093 | 0.358 |
Number of reflections | 26573 | |
<I/σ(I)> | 10.3 | 6.6 |
Completeness [%] | 99.0 | 97.7 |
Redundancy | 5.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.2M potassium sodium tartrate, 20%W/V polyethylene glycol 3350, 19mg/ml protein concentration, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |