2ZKG
Crystal structure of unliganded SRA domain of mouse Np95
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.334, 67.013, 69.572 |
| Unit cell angles | 91.11, 65.01, 84.48 |
Refinement procedure
| Resolution | 29.310 - 1.770 |
| R-factor | 0.18797 |
| Rwork | 0.186 |
| R-free | 0.21678 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bi7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.401 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.071 | 0.372 |
| Number of reflections | 96644 | |
| Completeness [%] | 95.4 | 77.3 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1M Tris-HCl (pH8.0), 0.1M Sodium acetate, 12% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
