2ZKA
Urate oxidase complexed with 8-azaxanthine under 1.0 MPa oxygen pressure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2005-11-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97625 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 80.172, 96.227, 105.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.970 - 1.610 |
R-factor | 0.17374 |
Rwork | 0.173 |
R-free | 0.18972 |
Structure solution method | rigid body |
Starting model (for MR) | 2iba |
RMSD bond length | 0.011 |
RMSD bond angle | 1.488 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.670 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.054 | 0.265 |
Number of reflections | 52656 | |
<I/σ(I)> | 12.5 | 3 |
Completeness [%] | 99.4 | 99 |
Redundancy | 4.6 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 10mg/ml urate oxidase, 0.2mg/ml 8-azaxanthine, 50mM Tris, 20mM NaCl, PEG 8000 4-10%, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |