2ZJR
Refined native structure of the large ribosomal subunit (50S) from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 169.900, 408.900, 694.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.900 - 2.910 |
Rwork | 0.277 |
R-free | 0.31100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nkw |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.910 | 3.040 |
High resolution limit [Å] | 2.910 | 2.910 |
Number of reflections | 493787 | |
Completeness [%] | 94.1 | 73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |