2ZJC
TNFR1 selectve TNF mutant; R1-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-13 |
| Detector | ADSC |
| Spacegroup name | H 3 |
| Unit cell lengths | 135.873, 135.873, 58.023 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.670 - 2.500 |
| R-factor | 0.20881 |
| Rwork | 0.201 |
| R-free | 0.27222 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tnf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.271 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.103 | 0.530 |
| Number of reflections | 13445 | |
| <I/σ(I)> | 28.9 | 4.2 |
| Completeness [%] | 99.9 | 85.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH6.5 |
