2ZJ4

Isomerase domain of human glucose:fructose-6-phosphate amidotransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32B2
Synchrotron site	SPring-8
Beamline	BL32B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-08-04
Detector	RIGAKU RAXIS V
Spacegroup name	I 41 2 2
Unit cell lengths	78.927, 78.927, 277.323
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.000 - 2.200
R-factor	0.16
Rwork	0.158
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zj3
RMSD bond length	0.021
RMSD bond angle	1.778
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.530
High resolution limit [Å]	2.200
Number of reflections	22501
Completeness [%]	98.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	12% Isoporpanol, 0.04M Tris, 0.08M Ammonium acetate, 12% glycerol 10-fold excess of 2-deoxy-2-aminoglucitol-6-phosphate, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K