2ZJ4
Isomerase domain of human glucose:fructose-6-phosphate amidotransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32B2 |
Synchrotron site | SPring-8 |
Beamline | BL32B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-08-04 |
Detector | RIGAKU RAXIS V |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 78.927, 78.927, 277.323 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.000 - 2.200 |
R-factor | 0.16 |
Rwork | 0.158 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zj3 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.778 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.530 |
High resolution limit [Å] | 2.200 |
Number of reflections | 22501 |
Completeness [%] | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 12% Isoporpanol, 0.04M Tris, 0.08M Ammonium acetate, 12% glycerol 10-fold excess of 2-deoxy-2-aminoglucitol-6-phosphate, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K |