2ZIF
Crystal Structure of TTHA0409, Putative DNA Modification Methylase from Thermus thermophilus HB8- Complexed with S-Adenosyl-L-Methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2008-02-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.900, 58.636, 81.224 |
Unit cell angles | 90.00, 106.06, 90.00 |
Refinement procedure
Resolution | 40.670 - 2.400 |
Rwork | 0.217 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zie |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.041 | 0.320 |
Number of reflections | 22675 | |
<I/σ(I)> | 29.7 | 3.85 |
Completeness [%] | 99.4 | 99.3 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 0.1M Tris HCl, 42% Ethanol, 25mM Mg Chloride, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |