2ZI4
C4S dCK variant of dCK in complex with L-dA+ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.730, 79.730, 93.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.18693 |
| Rwork | 0.179 |
| R-free | 0.25680 |
| Structure solution method | Rigid body refinement |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.572 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.870 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.111 | 0.346 |
| Number of reflections | 30274 | |
| <I/σ(I)> | 13.9 | 6 |
| Completeness [%] | 99.5 | 97.6 |
| Redundancy | 9.1 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.0M Sodium Citrate, 100mM Hepes, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
