2ZHX
Crystal structure of Uracil-DNA Glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.143, 64.274, 203.677 |
| Unit cell angles | 90.00, 109.72, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| R-factor | 0.23581 |
| Rwork | 0.234 |
| R-free | 0.27638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ugh 1UUG and 1UDI |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.435 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 43788 | |
| <I/σ(I)> | 8.2 | 2.4 |
| Completeness [%] | 97.2 | 91.8 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 10%(w/v) PEG 8000 and 0.2M NaCl in 0.1M phosphate buffer pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
