2ZGH
Crystal Structure of active granzyme M bound to its product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.598, 74.598, 112.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.030 - 2.170 |
| R-factor | 0.21393 |
| Rwork | 0.212 |
| R-free | 0.25038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zgc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.641 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.049 | 0.448 |
| Number of reflections | 19590 | |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Bicine, 0.2M Li2SO4, 20% PEG3350, pH8.50, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
