2ZGC
Crystal Structure of Active Human Granzyme M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2007-07-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.391, 74.391, 113.199 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.560 - 1.960 |
| R-factor | 0.21166 |
| Rwork | 0.209 |
| R-free | 0.25701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dst |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.726 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.065 | 0.340 |
| Number of reflections | 26319 | |
| <I/σ(I)> | 28 | 2.08 |
| Completeness [%] | 98.5 | 92.1 |
| Redundancy | 6.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M BICINE, 0.2M Li2SO4, 0.1M MGCL2, 19% PEG3350, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
