2Z32
Crystal structure of Keap1 complexed with Prothymosin alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2006-05-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 103.515, 103.515, 56.160 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.16879 |
Rwork | 0.166 |
R-free | 0.22317 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDN ENTRY 1X2J |
RMSD bond length | 0.017 |
RMSD bond angle | 1.619 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.074 | 0.228 |
Number of reflections | 21733 | |
Completeness [%] | 93.1 | 99.8 |
Redundancy | 10.6 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | Ammonium sulfate, Li2SO4, Na Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |